• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

AGXT2 Monoclonal antibody

AGXT2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A5B12

Cat No : 66602-1-Ig

Print datasheet

Synonyms

AGT 2, AGT2, AGXT2, Beta ALAAT II, D AIBAT

验证数据展示

  • WB analysis using 66602-1-Ig

    WB analysis using 66602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66602-1-Ig (AGXT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66602-1-Ig

    IHC staining of human kidney using 66602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66602-1-Ig (AGXT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66602-1-Ig

    IHC staining of human kidney using 66602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66602-1-Ig (AGXT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66602-1-Ig targets AGXT2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen AGXT2 fusion protein Ag26888 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alanine-glyoxylate aminotransferase 2
Synonyms AGT 2, AGT2, AGXT2, Beta ALAAT II, D AIBAT
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_001306173
Gene Symbol AGXT2
Gene ID (NCBI) 64902
RRIDAB_2881962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGXT2 antibody 66602-1-IgDownload protocol
IHC protocol for AGXT2 antibody 66602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols