ALK Monoclonal antibody

ALK Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

7G9E3

Cat No : 60321-1-Ig

Print datasheet

Synonyms

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK



经过测试的应用

Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

60321-1-Ig targets ALK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ALK fusion protein Ag21493 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name anaplastic lymphoma receptor tyrosine kinase
Synonyms ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK
Calculated Molecular Weight 1620 aa, 176 kDa
GenBank Accession NumberNM_004304
Gene Symbol ALK
Gene ID (NCBI) 238
RRIDAB_2881432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UM73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).

实验方案

Product Specific Protocols
IHC protocol for ALK antibody 60321-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur J Med Chem

Discovery of 2,4-pyrimidinediamine derivatives as potent dual inhibitors of ALK and HDAC.

Authors - Tao Pan
humanWB

Neoplasma

miR-1271 inhibits OSCC cell growth and metastasis by targeting ALK.

Authors - D Kong
humanWB

iScience

DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer

Authors - Qingwen Huang