ARL13B Monoclonal antibody

ARL13B Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Canine, Human, mouse, pig and More (2)

Applications

IF, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H6C3

Cat No : 66739-1-Ig

Print datasheet

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8



经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, NCCIT cell
Positive IF detected inMDCK cells, mouse heart tissue, hTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66739-1-Ig targets ARL13B in WB, IF, ELISA applications and shows reactivity with Canine, Human, mouse, pig samples.

Tested Applications IF, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity Canine, Human, mouse, pig
Cited Reactivityhuman, mouse, rat
Immunogen ARL13B fusion protein Ag12031 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor-like 13B
Synonyms ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2882088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

实验方案

Product Specific Protocols
WB protocol for ARL13B antibody 66739-1-IgDownload protocol
IF protocol for ARL13B antibody 66739-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,WB

Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda
humanWB

Elife

Multiple ciliary localization signals control INPP5E ciliary targeting

Authors - Dario Cilleros-Rodriguez
humanIF

Sci Rep

Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.

Authors - Elisa Molinari
ratIF

eNeuro

CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity.

Authors - Yasmin H Ramadan
humanIF

PLoS One

Embryonic and foetal expression patterns of the ciliopathy gene CEP164.

Authors - L A Devlin
mouseIF

Life Sci Alliance

HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails.

Authors - Pablo Barbeito