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  • KD/KO Validated

ARL2BP Polyclonal antibody

ARL2BP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10090-2-AP

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Synonyms

ARF like 2 binding protein, ARL2BP, BART, BART1, Binder of ARF2 protein 1



经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10090-2-AP targets ARL2BP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited Applications IF, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ARL2BP fusion protein Ag0138 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor-like 2 binding protein
Synonyms ARF like 2 binding protein, ARL2BP, BART, BART1, Binder of ARF2 protein 1
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC003087
Gene Symbol ARL2BP
Gene ID (NCBI) 23568
RRIDAB_2058518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.

实验方案

Product Specific Protocols
WB protocol for ARL2BP antibody 10090-2-APDownload protocol
IHC protocol for ARL2BP antibody 10090-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Am J Hum Genet

Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.

Authors - Davidson Alice E AE
  • KD Validated
humanWB

Cancer Res

Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.

Authors - Keisuke Taniuchi
humanWB,IF

Neoplasia

BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.

Authors - Taniuchi Keisuke K
  • KD Validated
humanWB,IF

PLoS One

BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.

Authors - Taniuchi Keisuke K
  • KD Validated
humanWB,IF

Invest Ophthalmol Vis Sci

Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.

Authors - Mori Naoki N
humanWB,IF,IP

Int J Oncol

BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.

Authors - Taniuchi Keisuke K