BBS9 Polyclonal antibody

BBS9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14460-1-AP

Print datasheet

Synonyms

B1, Bardet Biedl syndrome 9, BBS9, C18, D1, Protein PTHB1, PTHB1



经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue, human heart tissue, HeLa cells, Jurkat cells, mouse heart tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14460-1-AP targets BBS9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen BBS9 fusion protein Ag5665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Bardet-Biedl syndrome 9
Synonyms B1, Bardet Biedl syndrome 9, BBS9, C18, D1, Protein PTHB1, PTHB1
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032715
Gene Symbol BBS9
Gene ID (NCBI) 27241
RRIDAB_10598001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SYG4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BBS9 antibody 14460-1-APDownload protocol
IHC protocol for BBS9 antibody 14460-1-APDownload protocol
IP protocol for BBS9 antibody 14460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
humanWB

EMBO Rep

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

Authors - Benjamin Vitre
mouseWB

Invest Ophthalmol Vis Sci

Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.

Authors - Satyabrata Sinha