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C21orf2 Polyclonal antibody

C21orf2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27609-1-AP

Print datasheet

Synonyms

A2, C21orf HUMF09G8.5, C21orf2, YF5, YF5/A2

验证数据展示

  • WB analysis using 27609-1-AP

    WB analysis using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27609-1-AP

    IHC staining of human lung cancer using 27609-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293T using 27609-1-AP

    IP experiment of HEK-293T using 27609-1-AP

    IP result of anti-C21orf2 (IP:27609-1-AP, 4ug; Detection:27609-1-AP 1:3000) with HEK-293T cells lysate 1320 ug.

经过测试的应用

Positive WB detected inHEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27609-1-AP targets C21orf2 in WB, IP, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity Human
Cited Reactivityhuman
Immunogen C21orf2 fusion protein Ag26470 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 21 open reading frame 2
Synonyms A2, C21orf HUMF09G8.5, C21orf2, YF5, YF5/A2
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC072012
Gene Symbol C21orf2
Gene ID (NCBI) 755
RRIDAB_2880924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43822
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C21orf2 antibody 27609-1-APDownload protocol
IHC protocol for C21orf2 antibody 27609-1-APDownload protocol
IP protocol for C21orf2 antibody 27609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article