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  • Featured Product
  • KD/KO Validated

CaMKII alpha-Specific Polyclonal antibody

CaMKII alpha-Specific Polyclonal Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC,IF-P, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20666-1-AP

Print datasheet

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, CaMKII alpha, CaMKII alpha-Specific, KIAA0968

验证数据展示

  • WB analysis of HEK-293 using 20666-1-AP

    WB analysis of HEK-293 using 20666-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20666-1-AP (CaMKII alpha-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20666-1-AP

    IP experiment of HEK-293 using 20666-1-AP

    IP result of anti-CaMKII alpha-Specific (IP:20666-1-AP, 4ug; Detection:20666-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

  • IHC staining of human brain using 20666-1-AP

    IHC staining of human brain using 20666-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20666-1-AP (CaMKII alpha-Specific antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 20666-1-AP

    IHC staining of human brain using 20666-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20666-1-AP (CaMKII alpha-Specific antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse brain using 20666-1-AP

    IF Staining of mouse brain using 20666-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20666-1-AP (CaMKII alpha-Specific antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, SH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20666-1-AP targets CaMKII alpha-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC,IF-P, IHC, IP, WB, ELISA
Cited ApplicationsCoIP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II alpha
Synonyms CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, CaMKII alpha, CaMKII alpha-Specific, KIAA0968
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_015981
Gene Symbol CAMK2A
Gene ID (NCBI) 815
RRIDAB_2878722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII alpha, also named CAMKA and KIAA0968, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody is specific to CAMK2A.

实验方案

Product Specific Protocols
WB protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IHC protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IF protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
IP protocol for CaMKII alpha-Specific antibody 20666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
  • KD Validated
View Article
WB

ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu
View Article
WB

Sci Total Environ

Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.

Authors - Huanliang Liu
View Article
humanWB

Int J Biol Sci

Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression.

Authors - Qing Liu
View Article
WB

Gerontology

Proteomic Profile of Carbonylated Proteins Screen the Regulation of Calmodulin-Dependent Protein Kinases-AMPK-Beclin1 in Aerobic Exercise-Induced Autophagy in Middle-Aged Rat Hippocampus.

Authors - Wenfeng Liu
View Article
WB

J Neuropathol Exp Neurol

Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.

Authors - Wenfeng Liu
View Article