• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CCDC8 Polyclonal antibody

CCDC8 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 27194-1-AP

Print datasheet

Synonyms

CCDC8

验证数据展示

  • IP experiment of HEK-293 using 27194-1-AP

    IP experiment of HEK-293 using 27194-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27194-1-AP (CCDC8 antibody) at dilution of 1:300.

  • WB analysis of HEK-293 using 27194-1-AP

    WB analysis of HEK-293 using 27194-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27194-1-AP (CCDC8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27194-1-AP

    IHC staining of human lung cancer using 27194-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27194-1-AP (CCDC8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27194-1-AP

    IHC staining of human lung cancer using 27194-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27194-1-AP (CCDC8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27194-1-AP is CCDC8 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen CCDC8 fusion protein Ag25794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 8
Synonyms CCDC8
Observed Molecular Weight 90 kDa, 59 kDa
GenBank Accession NumberBC025243
Gene Symbol CCDC8
Gene ID (NCBI) 83987
RRIDAB_2880795
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0W5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled-coil domain-containing protein 8 (CCDC8) is a 538-amino acid protein with the molecular mass of 59kD and 90kD cause of the posttranslational modifications including amidation, glycosylation, phosphorylation, and myristalation (PMID: 21737058). CCDC8 has 2 coiled-coil domains, and the proteins containing this domain are involved in diverse biological processes, such as the regulation of gene expression, cell division, and membrane fusion. CCDC8 is evolutionarily conserved, and CCDC8 mutation leads to the development of 3M syndrome in humans, a primordial growth disorder, by interacting with CUL7, OBSL1, and P53. Low-level or no expression of CCDC8 was shown to be closely related to the development of some tumors, such as renal cell carcinoma, multiple myeloma, breast cancer, and prostate cancer (PMID: 21737058, 27342910).

实验方案

Product Specific Protocols
WB protocol for CCDC8 antibody 27194-1-APDownload protocol
IHC protocol for CCDC8 antibody 27194-1-APDownload protocol
IP protocol for CCDC8 antibody 27194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols