CD107a / LAMP1 Polyclonal antibody
CD107a / LAMP1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, U937 cells |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue, human gliomas tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21997-1-AP targets CD107a / LAMP1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | FC, IHC, IP, WB |
| Tested Reactivity | human |
| Cited Reactivity | human, turtle, monkey, zebrafish, bovine |
| Immunogen | CD107a / LAMP1 fusion protein Ag17367 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lysosomal-associated membrane protein 1 |
| Synonyms | CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker |
| Calculated Molecular Weight | 417 aa, 45 kDa |
| Observed Molecular Weight | 90-120 kDa |
| GenBank Accession Number | BC021288 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2878966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| IHC protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis. | ||
Adv Sci (Weinh) Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy | ||
Arthritis Rheumatol LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands | ||
Mol Ther Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase | ||
Nat Commun Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer. | ||
Sci Total Environ TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles |
