CD14 Polyclonal antibody
CD14 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, RAW 264.7 cells, human spleen tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human appendicitis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | RAW 264.7 cells |
| Positive FC detected in | RAW 264.7 cells |
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17000-1-AP targets CD14 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | CD14 fusion protein Ag10693 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD14 molecule |
| Synonyms | CD14, CD14 molecule |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC010507 |
| Gene Symbol | CD14 |
| Gene ID (NCBI) | 929 |
| ENSEMBL Gene ID | ENSG00000170458 |
| RRID | AB_2074048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08571 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD14 antibody 17000-1-AP | Download protocol |
| IHC protocol for CD14 antibody 17000-1-AP | Download protocol |
| IF protocol for CD14 antibody 17000-1-AP | Download protocol |
| FC protocol for CD14 antibody 17000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Immunol Clostridium septicum α-toxin activates the NLRP3 inflammasome by engaging GPI-anchored proteins. | ||
Nat Commun HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis. | ||
EBioMedicine Patient-derived melanoma organoid models facilitate the assessment of immunotherapies | ||
Biomaterials Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury | ||
Biomaterials Cerebro- and renoprotective activities through platelet-derived biomaterials against cerebrorenal syndrome in rat model. |
