Complement factor B Monoclonal antibody
Complement factor B Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
8D2B6
验证数据展示
经过测试的应用
| Positive WB detected in | human blood tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver tissue |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 66154-1-Ig is Complement factor B Fusion Protein expressed in E. coli.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Tested Reactivity | human |
| Immunogen | Complement factor B fusion protein Ag19107 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | complement factor B |
| Synonyms | BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC007990 |
| Gene Symbol | CFB |
| Gene ID (NCBI) | 629 |
| RRID | AB_2881550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | P00751 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Complement factor B antibody 66154-1-Ig | Download protocol |
| IHC protocol for Complement factor B antibody 66154-1-Ig | Download protocol |
| IF protocol for Complement factor B antibody 66154-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
