CHAT Polyclonal antibody

CHAT Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24418-1-AP

Print datasheet

Synonyms

CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2



经过测试的应用

Positive WB detected inrat brain tissue, human placenta tissue, mouse brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 3 publications below

产品信息

The immunogen of 24418-1-AP is CHAT Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IF
Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Immunogen CHAT fusion protein Ag19550 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name choline acetyltransferase
Synonyms CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2
Calculated Molecular Weight 748 aa, 83 kDa
Observed Molecular Weight 60-66 kDa, 45 kDa
GenBank Accession NumberBC130617
Gene Symbol CHAT
Gene ID (NCBI) 1103
RRIDAB_2879536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively.

实验方案

Product Specific Protocols
WB protocol for CHAT antibody 24418-1-APDownload protocol
IHC protocol for CHAT antibody 24418-1-APDownload protocol
IF protocol for CHAT antibody 24418-1-APDownload protocol
IP protocol for CHAT antibody 24418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
mouseIF

bioRxiv

Expression of the ACE2 virus entry protein in the nervus terminalis reveals the potential for an alternative route to brain infection in COVID-19.

Authors - Katarzyna Bilinska
mouseIF

Neurobiol Dis

NRF2 activation suppresses motor neuron ferroptosis induced by the SOD1G93A mutation and exerts neuroprotection in amyotrophic lateral sclerosis

Authors - Biying Yang