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- KD/KO Validated
CPT1A Polyclonal antibody
CPT1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
298
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15184-1-AP targets CPT1A in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, FC, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, yellow croaker, zebrafish, pig, duck, bovine |
Immunogen | CPT1A fusion protein Ag7202 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | carnitine palmitoyltransferase 1A (liver) |
Synonyms | CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1 |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 86 kDa |
GenBank Accession Number | BC000185 |
Gene Symbol | CPT1A |
Gene ID (NCBI) | 1374 |
RRID | AB_2084676 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P50416 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CPT1A antibody 15184-1-AP | Download protocol |
IHC protocol for CPT1A antibody 15184-1-AP | Download protocol |
IF protocol for CPT1A antibody 15184-1-AP | Download protocol |
IP protocol for CPT1A antibody 15184-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Cell Metab Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis. | ||
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Cell Metab SGLT2 Inhibition Mediates Protection from Diabetic Kidney Disease by Promoting Ketone Body-Induced mTORC1 Inhibition. |