Alpha E-Catenin Polyclonal antibody

Alpha E-Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12831-1-AP

Print datasheet

Synonyms

alpha catenin, Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, α-catenin, α-E-catenin, αE-catenin



经过测试的应用

Positive WB detected inmouse uterus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CTNNA1 Ready-To-Use IHC Kit. CTNNA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12831-1-AP targets Alpha E-Catenin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Alpha E-Catenin fusion protein Ag3565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Synonyms alpha catenin, Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, α-catenin, α-E-catenin, αE-catenin
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC031262
Gene Symbol CTNNA1
Gene ID (NCBI) 1495
RRIDAB_2087822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

实验方案

Product Specific Protocols
WB protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IHC protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IF protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IP protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
FC protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.

Authors - Q Zhou
mouseIF

Elife

Selective YAP activation in Procr cells is essential for ovarian stem/progenitor expansion and epithelium repair.

Authors - Jingqiang Wang
humanWB

Oncogene

The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.

Authors - Quan Zhou
humanWB

Helicobacter

Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.

Authors - Chen Zhang
humanWB

Cells

P-Cadherin Regulates Intestinal Epithelial Cell Migration and Mucosal Repair, but Is Dispensable for Colitis Associated Colon Cancer.

Authors - Nayden G Naydenov
humanIF

Sci Rep

SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth.

Authors - Rehab Alshargabi