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  • KD/KO Validated

CUL2 Polyclonal antibody

CUL2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10981-2-AP

Print datasheet

Synonyms

CUL 2, CUL2, cullin 2



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10981-2-AP targets CUL2 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsCoIP, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CUL2 fusion protein Ag1434 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cullin 2
Synonyms CUL 2, CUL2, cullin 2
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC009591
Gene Symbol CUL2
Gene ID (NCBI) 8453
RRIDAB_2086422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13617
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

实验方案

Product Specific Protocols
WB protocol for CUL2 antibody 10981-2-APDownload protocol
IHC protocol for CUL2 antibody 10981-2-APDownload protocol
IP protocol for CUL2 antibody 10981-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Authors - Manuela Antonioli
  • KD Validated
humanWB

Cell Death Differ

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.

Authors - Pingzhao Zhang
humanWB

Oncogene

iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.

Authors - Dong Zhao
humanWB

Aging (Albany NY)

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.

Authors - Xiao Dong
  • KD Validated
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H
  • KD Validated
humanWB

J Cell Sci

Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.

Authors - Tomoaki Nagai
  • KD Validated