DEFA6 Polyclonal antibody

DEFA6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17923-1-AP

Print datasheet

Synonyms

DEF6, DEFA6, Defensin 6, Defensin, alpha 6, defensin, alpha 6, Paneth cell, HD 6



经过测试的应用

Positive WB detected inCOLO 320 cells, mouse small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DEFA6 Ready-To-Use IHC Kit. DEFA6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

17923-1-AP targets DEFA6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen DEFA6 fusion protein Ag12341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name defensin, alpha 6, Paneth cell-specific
Synonyms DEF6, DEFA6, Defensin 6, Defensin, alpha 6, defensin, alpha 6, Paneth cell, HD 6
Calculated Molecular Weight 100 aa, 11 kDa
GenBank Accession NumberBC093951
Gene Symbol DEFA6
Gene ID (NCBI) 1671
RRIDAB_2878468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01524
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. DEFA6 is alpha 6 of Defensin. This protein is secret expression and can be cleaved in to the fragments with MW 4-6 kDa. It can be used as a maker of Paneth cell. DEFA6 has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. it may protect cells against infection with HIV-1.

实验方案

Product Specific Protocols
WB protocol for DEFA6 antibody 17923-1-APDownload protocol
IHC protocol for DEFA6 antibody 17923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
HumanIF

J Clin Invest

Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity.

Authors - Kathrin I Liszt