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DYNC1LI1 Polyclonal antibody

DYNC1LI1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25326-1-AP

Print datasheet

Synonyms

DLC A, DNCLI1, DYNC1LI1, Dynein light chain A, LIC1

验证数据展示

  • IHC staining of mouse pancreas using 25326-1-AP

    IHC staining of mouse pancreas using 25326-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25326-1-AP (DYNC1LI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 25326-1-AP

    IHC staining of mouse pancreas using 25326-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25326-1-AP (DYNC1LI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 25326-1-AP

    WB analysis of K-562 using 25326-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25326-1-AP (DYNC1LI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25326-1-AP

    IF Staining of HeLa using 25326-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25326-1-AP (DYNC1LI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25326-1-AP targets DYNC1LI1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen DYNC1LI1 fusion protein Ag18191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, cytoplasmic 1, light intermediate chain 1
Synonyms DLC A, DNCLI1, DYNC1LI1, Dynein light chain A, LIC1
Calculated Molecular Weight 523 aa, 57 kDa
Observed Molecular Weight 57-60 kDa
GenBank Accession NumberBC131620
Gene Symbol DYNC1LI1
Gene ID (NCBI) 51143
RRIDAB_2880031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6G9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DYNC1LI1 antibody 25326-1-APDownload protocol
IHC protocol for DYNC1LI1 antibody 25326-1-APDownload protocol
IF protocol for DYNC1LI1 antibody 25326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article