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  • KD/KO Validated

Desmoplakin Polyclonal antibody

Desmoplakin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25318-1-AP

Print datasheet

Synonyms

desmoplakin, Desmoplakin I, Desmoplakin II, DP, DPI, DPII, DSP



经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, mouse heart tissue, BxPC-3 cells, HEK-293 cells
Positive IP detected inBxPC-3 cells
Positive IHC detected inrat heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, rat heart tissue
Positive FC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25318-1-AP targets Desmoplakin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Desmoplakin fusion protein Ag18065 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name desmoplakin
Synonyms desmoplakin, Desmoplakin I, Desmoplakin II, DP, DPI, DPII, DSP
Calculated Molecular Weight 2871 aa, 332 kDa
Observed Molecular Weight 240-330 kDa
GenBank Accession NumberBC140802
Gene Symbol DSP
Gene ID (NCBI) 1832
RRIDAB_2880028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15924
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

实验方案

Product Specific Protocols
WB protocol for Desmoplakin antibody 25318-1-APDownload protocol
IHC protocol for Desmoplakin antibody 25318-1-APDownload protocol
IF protocol for Desmoplakin antibody 25318-1-APDownload protocol
IP protocol for Desmoplakin antibody 25318-1-APDownload protocol
FC protocol for Desmoplakin antibody 25318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bone Res

Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone.

Authors - Shengzhi Liu
mouseWB,IF

Theranostics

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Authors - Min Zhang
humanWB

Cancer Lett

ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner.

Authors - Liang Xu
mouseWB

Cancers (Basel)

Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium.

Authors - Tomohiko Sano
humanWB

J Cell Mol Med

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Authors - Lin-Jian Wang
  • KD Validated
humanWB

Cell Death Discov

Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway.

Authors - Yanlei Yang
  • KD Validated