HuA/B/C/D Polyclonal antibody

HuA/B/C/D Polyclonal Antibody for IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF-P, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13032-1-AP

Print datasheet

Synonyms

ELAV like protein 4, ELAVL4, Hu antigen D, Hu C/D, HuA/B/C/D, HUD, PNEM



经过测试的应用

Positive WB detected inhuman cerebellum tissue, mouse cerebellum tissue, PC-13 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13032-1-AP targets HuA/B/C/D in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen HuA/B/C/D fusion protein Ag3678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Synonyms ELAV like protein 4, ELAVL4, Hu antigen D, Hu C/D, HuA/B/C/D, HUD, PNEM
Calculated Molecular Weight 380 aa, 42 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC036071
Gene Symbol ELAVL4
Gene ID (NCBI) 1996
RRIDAB_2096385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].

实验方案

Product Specific Protocols
WB protocol for HuA/B/C/D antibody 13032-1-APDownload protocol
IHC protocol for HuA/B/C/D antibody 13032-1-APDownload protocol
IF protocol for HuA/B/C/D antibody 13032-1-APDownload protocol
IP protocol for HuA/B/C/D antibody 13032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

J Neurosci Methods

Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF.

Authors - Ruth Butler-Ryan
humanIHC,WB

J Cosmet Dermatol

Study on the correlation of high expression level of HuD with seborrheic keratosis and skin aging

Authors - Juping Chen
mouseIF

Med Mol Morphol

Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T mice

Authors - Nami O Yamada
mouseIF

Biomed Pharmacother

Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axis

Authors - Jinzhao Bai