EWS Polyclonal antibody

EWS Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55191-1-AP

Print datasheet

Synonyms

EWS, EWS oncogene, EWSR1, RNA binding protein EWS



经过测试的应用

Positive WB detected inK-562 cells, mouse testis tissue, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EWSR1/EWS Ready-To-Use IHC Kit. EWSR1/EWS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55191-1-AP targets EWS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ewing sarcoma breakpoint region 1
Synonyms EWS, EWS oncogene, EWSR1, RNA binding protein EWS
Calculated Molecular Weight 68 kDa
Observed Molecular Weight68-70 kDa
GenBank Accession NumberNM_005243
Gene Symbol EWSR1
Gene ID (NCBI) 2130
RRIDAB_10860907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).

实验方案

Product Specific Protocols
WB protocol for EWS antibody 55191-1-APDownload protocol
IHC protocol for EWS antibody 55191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

Nucleic Acids Res

The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.

Authors - Huiwu Ouyang
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
ratWB

Mol Ther Nucleic Acids

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.

Authors - Zhijie Shen
mouseIF

J Reprod Dev

Lipid droplets synthesized during luteinization are degraded after pregnancy

Authors - Junichiro Mitsui