FBL Monoclonal antibody
FBL Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse and More (1)
Applications
FC, IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3A9E8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 1 publications below |
产品信息
66985-1-Ig targets FBL in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | IF, WB |
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human |
Immunogen | FBL fusion protein Ag8841 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fibrillarin |
Synonyms | FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1 |
Calculated Molecular Weight | 321 aa, 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC019260 |
Gene Symbol | FBL |
Gene ID (NCBI) | 2091 |
RRID | AB_2882303 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P22087 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for FBL antibody 66985-1-Ig | Download protocol |
IHC protocol for FBL antibody 66985-1-Ig | Download protocol |
IF protocol for FBL antibody 66985-1-Ig | Download protocol |
IP protocol for FBL antibody 66985-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
BMC Biol A pair of long intergenic non-coding RNA LINC00887 variants act antagonistically to control Carbonic Anhydrase IX transcription upon hypoxia in tongue squamous carcinoma progression | ||
Thorac Cancer LncRNA FAM83A-AS1 promotes lung adenocarcinoma progression by enhancing the pre-mRNA stability of FAM83A. |