FIS1 Monoclonal antibody

FIS1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (5)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F10E1

Cat No : 66635-1-Ig

Print datasheet

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11



经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66635-1-Ig targets FIS1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, goat, fish
Immunogen FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_2881994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
WB protocol for FIS1 antibody 66635-1-IgDownload protocol
IHC protocol for FIS1 antibody 66635-1-IgDownload protocol
IF protocol for FIS1 antibody 66635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
mouseWB

Front Immunol

Metformin Modulates T Cell Function and Alleviates Liver Injury Through Bioenergetic Regulation in Viral Hepatitis.

Authors - Lanman Xu
fishWB

J Physiol Sci

Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart

Authors - Zhanglin Chen
humanIF

Biol Pharm Bull

Ampelopsin inhibits breast cancer cell growth through mitochondrial apoptosis pathway

Authors - YUE LI
ratIHC

J Mol Histol

Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats

Authors - Fan Wang
ratWB

Aging (Albany NY)

Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF

Authors - Xiao-Lin Jiang