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FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10060-1-AP

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Synonyms

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human brain tissue, human testis tissue, Jurkat cells, MCF-7 cells, T-47D cells, MDA-MB-453s cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10060-1-AP targets FKBPL in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited Applications ChIP, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen FKBPL fusion protein Ag0112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein like
Synonyms DIR1, FK506 binding protein like, FKBPL, NG7, WISP39
Calculated Molecular Weight 349 aa, 38 kDa
Observed Molecular Weight 42-48 kDa
GenBank Accession NumberBC004168
Gene Symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2262677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193). It can be detected the band between 38 kDa and 48 kDa by western blot.

实验方案

Product Specific Protocols
WB protocol for FKBPL antibody 10060-1-APDownload protocol
IHC protocol for FKBPL antibody 10060-1-APDownload protocol
IP protocol for FKBPL antibody 10060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

J Adv Res

Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy

Authors - Jia-Jie Lin
humanWB

Cancer Res

FKBPL regulates estrogen receptor signaling and determines response to endocrine therapy.

Authors - McKeen Hayley D HD
humanIF

Cell Mol Life Sci

A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia

Authors - Sahar Masoumeh Ghorbanpour
humanWB

J Control Release

Delivery of RALA/siFKBPL nanoparticles via electrospun bilayer nanofibres: An innovative angiogenic therapy for wound repair.

Authors - Eoghan J Mulholland
  • KD Validated
humanWB,IHC

Clin Cancer Res

FKBPL and peptide derivatives: novel biological agents that inhibit angiogenesis by a CD44-dependent mechanism.

Authors - Valentine Andrea A
humanWB, ChIP

Oncogene

Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor growth and response to tamoxifen.

Authors - C Donley