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FOXM1 Polyclonal antibody
FOXM1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, HeLa cells, human testis tissue, mouse thymus tissue, mouse colon tissue, mouse testis tissue, HEK-293 cells, mouse spleen tissue, L02 cells, MCF-7 cells, A431 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human pancreas cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13147-1-AP targets FOXM1 in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB,RIP,IP,IHC,IF,ChIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | FOXM1 fusion protein Ag3729 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | forkhead box M 1 |
| Synonyms | FKHL16, forkhead box M1, FOXM1, FOXM1B, HFH 11, HFH11, HNF 3, HNF 3/fork head homolog 11, INS 1, M phase phosphoprotein 2, MPHOSPH2, MPP 2, MPP2, PIG29, TGT3, Transcription factor Trident, TRIDENT, WIN |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 84-110 kDa |
| GenBank Accession Number | BC006192 |
| Gene Symbol | FOXM1 |
| Gene ID (NCBI) | 2305 |
| RRID | AB_2106213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| IHC protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| IP protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.
| ||
Nat Commun K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis | ||
Acta Pharm Sin B Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation. | ||
Nat Commun Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation. | ||
Nat Commun FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence. | ||
Nat Commun circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.
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