GAMT Polyclonal antibody
GAMT Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, rat liver tissue, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human liver tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
10880-1-AP targets GAMT in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat, zebrafish |
| Immunogen | GAMT fusion protein Ag1327 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | guanidinoacetate N-methyltransferase |
| Synonyms | GAMT, PIG2, TP53I2 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC016760 |
| Gene Symbol | GAMT |
| Gene ID (NCBI) | 2593 |
| RRID | AB_2109304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GAMT antibody 10880-1-AP | Download protocol |
| IHC protocol for GAMT antibody 10880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. | ||
Toxicol Appl Pharmacol Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B. | ||
Ann Transl Med Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury. | ||
Brain Res GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain. | ||
J Fish Biol Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio. | ||
Redox Biol Unbalanced redox status network as an early pathological event in congenital cataracts |
