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GAS7 Polyclonal antibody

GAS7 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10072-1-AP

Print datasheet

Synonyms

GAS 7, GAS7, growth arrest 7, KIAA0394, MLL/GAS7

验证数据展示

  • WB analysis of NIH/3T3 using 10072-1-AP

    WB analysis of NIH/3T3 using 10072-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10072-1-AP (GAS7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10072-1-AP

    IF Staining of HeLa using 10072-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10072-1-AP (GAS7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse testis tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10072-1-AP targets GAS7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Immunogen GAS7 fusion protein Ag0114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth arrest-specific 7
Synonyms GAS 7, GAS7, growth arrest 7, KIAA0394, MLL/GAS7
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC001152
Gene Symbol GAS7
Gene ID (NCBI) 8522
RRIDAB_2108090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60861
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth arrest-specific (GAS) genes are expressed preferentially in cells that enter a quiescent state. Gas7, first identified in serum-starved murine fibroblasts, encodes a 48-kDa protein. 3 isoforms of Gas7 were identified in rat brains, with molecular weight of 38, 47 and 55 kDa in SDS Page.

实验方案

Product Specific Protocols
WB protocol for GAS7 antibody 10072-1-APDownload protocol
IF protocol for GAS7 antibody 10072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biomater Sci

Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983-miR-6931-Gas7 interaction.

Authors - Wenchao Zhong
View Article
ratWB,IHC

J Proteome Res

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.

Authors - Ji Yuhua Y
View Article