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  • Featured Product
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GOSR2/Membrin Monoclonal antibody

GOSR2/Membrin Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7E7C7

Cat No : 66134-1-Ig

Print datasheet

Synonyms

27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin

验证数据展示

  • WB analysis of Jurkat using 66134-1-Ig

    WB analysis of Jurkat using 66134-1-Ig

    WB result of GOSR2/Membrin antibody (66134-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

  • WB analysis using 66134-1-Ig

    WB analysis using 66134-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66134-1-Ig

    IHC staining of human liver using 66134-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

  • IHC staining of human liver using 66134-1-Ig

    IHC staining of human liver using 66134-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

  • IF Staining of human liver cancer using 66134-1-Ig

    IF Staining of human liver cancer using 66134-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GOSR2/Membrin antibody (66134-1-Ig, Clone: 7E7C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, Jurkat cells, PC-12 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 66134-1-Ig is GOSR2/Membrin Fusion Protein expressed in E. coli.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen GOSR2/Membrin fusion protein Ag21393 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name golgi SNAP receptor complex member 2
Synonyms 27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin
Calculated Molecular Weight 212 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC009710
Gene Symbol GOSR2
Gene ID (NCBI) 9570
RRIDAB_2881533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

实验方案

Product Specific Protocols
WB protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
IHC protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
IF protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Authors - Dolma Choezom
View Article