GS28 Polyclonal antibody

GS28 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16106-1-AP

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Synonyms

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28



经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, human liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16106-1-AP targets GS28 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen GS28 fusion protein Ag9021 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi SNAP receptor complex member 1
Synonyms 28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28
Calculated Molecular Weight28.6 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC012620
Gene Symbol GOSR1
Gene ID (NCBI) 9527
RRIDAB_2294912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

实验方案

Product Specific Protocols
WB protocol for GS28 antibody 16106-1-APDownload protocol
IHC protocol for GS28 antibody 16106-1-APDownload protocol
IF protocol for GS28 antibody 16106-1-APDownload protocol
FC protocol for GS28 antibody 16106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols