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GSR Polyclonal antibody

GSR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 18257-1-AP

Print datasheet

Synonyms

GLUR, glutathione reductase, GR, GRase, GRD1, GSR



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse lung tissue, human placenta tissue, mouse testis tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18257-1-AP targets GSR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GSR fusion protein Ag13080 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione reductase
Synonyms GLUR, glutathione reductase, GR, GRase, GRD1, GSR
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC069244
Gene Symbol GSR
Gene ID (NCBI) 2936
RRIDAB_10598162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00390
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.

实验方案

Product Specific Protocols
WB protocol for GSR antibody 18257-1-APDownload protocol
IHC protocol for GSR antibody 18257-1-APDownload protocol
IF protocol for GSR antibody 18257-1-APDownload protocol
IP protocol for GSR antibody 18257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Redox Biol

Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.

Authors - Angel Gallego-Selles
mouseWB

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H
humanWB

Nutrients

A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle

Authors - Miriam Martinez-Canton
humanWB

Pharmacol Res

Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.

Authors - Meng-Ting Chang
humanWB

Free Radic Biol Med

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Authors - Kelei Dong
mouseWB

Free Radic Biol Med

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.

Authors - Jan Blecha