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HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

FC, IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10995-1-AP

Print datasheet

Synonyms

Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B



经过测试的应用

Positive WB detected inA431 cells, human urine exosomes tissue, A549 cells, HeLa cells, Jurkat cells, mouse brain, HEK-293 cells, mouse kidney, rat kidney
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells, HeLa cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy HSP70 Ready-To-Use IHC Kit. HSP70 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10995-1-AP targets HSP70 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications Cell treatment, CoIP, ELISA, FC, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, ostrich, oyster, pig, C. elegans, bovine
Immunogen HSP70 fusion protein Ag1446 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 1A
Synonyms Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC009322
Gene Symbol HSPA1A
Gene ID (NCBI) 3303
RRIDAB_2264230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DMV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

  1. What is Hsp70/HSP1A?

    HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

  2. FAQs for Hsp70

    a. I cannot detect Hsp70 by western blotting

    HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

    b. What loading control can I use for cellular stress experiments with Hsp70?

    Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

实验方案

Product Specific Protocols
WB protocol for HSP70 antibody 10995-1-APDownload protocol
IHC protocol for HSP70 antibody 10995-1-APDownload protocol
IF protocol for HSP70 antibody 10995-1-APDownload protocol
IP protocol for HSP70 antibody 10995-1-APDownload protocol
FC protocol for HSP70 antibody 10995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
humanWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KO Validated
humanWB

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao
mouseWB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
  • KD Validated
human,mouseWB,IHC

Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez