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HSP60 Polyclonal antibody

HSP60 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15282-1-AP

Print datasheet

Synonyms

HSP 60, HSP60, HSP65, HSPD1



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells, HeLa, mouse heart, rat heart
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15282-1-AP targets HSP60 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, ELISA, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, yeast, fish, pig, duck, rice, bovine
Immunogen HSP60 fusion protein Ag7401 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 60kDa protein 1 (chaperonin)
Synonyms HSP 60, HSP60, HSP65, HSPD1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC003030
Gene Symbol HSPD1
Gene ID (NCBI) 3329
RRIDAB_2121440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

实验方案

Product Specific Protocols
WB protocol for HSP60 antibody 15282-1-APDownload protocol
IHC protocol for HSP60 antibody 15282-1-APDownload protocol
IF protocol for HSP60 antibody 15282-1-APDownload protocol
IP protocol for HSP60 antibody 15282-1-APDownload protocol
FC protocol for HSP60 antibody 15282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
mouseWB

Nucleic Acids Res

RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis

Authors - Chao Liu
mouseWB

J Hazard Mater

Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy

Authors - Jun Wu
humanWB

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
mouseWB,IF

Nat Commun

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

Authors - Gustavo Della-Flora Nunes
humanWB

Nat Commun

EGFL9 promotes breast cancer metastasis by inducing cMET activation and metabolic reprogramming.

Authors - Fanyan Meng