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HTRA2 Polyclonal antibody

HTRA2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15775-1-AP

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Synonyms

HtrA serine peptidase 2, HTRA2, OMI, PARK13, PRSS25, Serine protease 25, Serine proteinase OMI



经过测试的应用

Positive WB detected inHeLa cells, human heart tissue, mouse skeletal muscle tissue, Raji cells, MCF-7 cells, Jurkat cells, HEK-293 cells, SH-SY5Y cells, COS-7 cells, Neuro-2a cells
Positive IP detected inRaji cells
Positive IHC detected inhuman kidney tissue, human colon tissue, human thyroid tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15775-1-AP targets HTRA2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, hamster
Immunogen HTRA2 fusion protein Ag8455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HtrA serine peptidase 2
Synonyms HtrA serine peptidase 2, HTRA2, OMI, PARK13, PRSS25, Serine protease 25, Serine proteinase OMI
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000096
Gene Symbol HTRA2
Gene ID (NCBI) 27429
RRIDAB_2122835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for HTRA2 antibody 15775-1-APDownload protocol
IHC protocol for HTRA2 antibody 15775-1-APDownload protocol
IP protocol for HTRA2 antibody 15775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cell Rep

TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria

Authors - Shiori Akabane
mouseWB

Int J Mol Sci

The Mechanism of Mitochondrial Injury in Alpha-1 Antitrypsin Deficiency Mediated Liver Disease.

Authors - Nazli Khodayari

Mol Neurobiol

Mitochondrial Proteome Changes Correlating with β-Amyloid Accumulation.

Authors - Katalin Völgyi
humanWB

Hum Mol Genet

Multi-OMICS study of a CHCHD10 variant causing ALS demonstrates metabolic rewiring and activation of endoplasmic reticulum and mitochondrial unfolded protein responses.

Authors - Isabella R Straub
humanWB

Cancer Sci

Establishment of MAGEC2-knockout cells and functional investigation of MAGEC2 in tumor cells.

Authors - Jingjing Wang
humanIHC

Cancer Biomark

Role of Smac, survivin, XIAP, and Omi/HtrA2 proteins in determining the chemotherapeutic response of patients with cervical cancer treated with neoadjuvant chemotherapy

Authors - Tianyi Ye