Human CD20 ELISA Kit

Catalog number: KE00082

规格

单价

96 T

¥2600

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Overview


Product name:
Human CD20 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
cell culture supernatant, cell lysate

Assay type:
Sandwich

Sensitivity:
0.33 ng/mL

Range:
0.78 ng/mL - 50 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell culture supernatant 98% 83%-122%
Cell lysate 101% 75%-121%

Product overview:
KE00082 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD20 ELISA kit is to be used to detect and quantify protein levels of endogenous CD20. The assay recognizes human CD20. A polyclonal antibody specific for CD20 has been pre-coated onto the microwells. The CD20 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD20 is added to detect the captured CD20 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
B lymphocyte antigen CD20, B1, Bp35, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7
Background

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.


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