Human CD31 ELISA Kit
Sensitivity
0.09 ng/mL
Range
0.25-16 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00041 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD31 ELISA kit is to be used to detect and quantify protein levels of endogenous CD31. The assay recognizes human CD31. A polyclonal antibody specific for CD31 has been pre-coated onto the microwells. The CD31 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD31 is added to detect the captured CD31 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human CD31 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5175 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 96% | 87-102% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. It is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. CD31 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell. As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis. Soluble form of CD31 is present in human plasma, resulting from alternative splicing of the transmembrane domain.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker |