Human CEACAM5 ELISA Kit

Catalog number: KE00042

规格

单价

96 T

¥2600

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Overview


Product name:
Human CEACAM5 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum,Plasma

Assay type:
Sandwich

Sensitivity:
32 pg/mL

Range:
125-8000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 94% 78-119%

Product overview:
KE00042 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CEACAM5 ELISA kit is to be used to detect and quantify protein levels of endogenous CEACAM5. The assay recognizes human CEACAM5. A polyclonal antibody specific for CEACAM5 has been pre-coated onto the microwells. The CEACAM5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CEACAM5 is added to detect the captured CEACAM5 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Carcinoembryonic antigen, CD66e, CEA, CEACAM5, DKFZp781M2392, Meconium antigen 100
Background

CEACAM5, also known as carcinoembryonic antigen (CEA) or CD66e, is a member of the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding. CEACAM5 is overexpressed in a wide variety of human cancers, including colon, breast, and lung. It inhibits anoikis and plays a role in tumorigenesis and metastasis. CEACAM5 is not usually present in the blood of healthy adults. However, it has been found that serum from people with colorectal, gastric, pancreatic, lung, and breast carcinoma has higher levels of CEACAM5 than healthy people. CEACAM5 is a tumor marker and is routinely exploited for diagnosis.


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