Human ICAM-1 ELISA Kit

Catalog number: KE00061

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Overview


Product name:
Human ICAM-1 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.02 ng/mL

Range:
0.156-10 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 93% 82%-113%
Cell culture supernatant 91% 82%-96%

Product overview:
KE00061 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ICAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous ICAM-1. The assay recognizes human ICAM-1. A polyclonal antibody specific for ICAM-1 has been pre-coated onto the microwells. The ICAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for ICAM-1 is added to detect the captured ICAM-1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58
Background

Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. Soluble form of ICAM-1 (sICAM-1) has been measured in various body fluids, with increased levels being found in serum from patients with cardiovascular disease, cancer and autoimmune diseases.


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