Human INS ELISA Kit

Catalog number: KE00045

规格

单价

96 T

¥2600

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Overview


Product name:
Human INS ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma

Assay type:
Sandwich

Sensitivity:
1.32 pmol/L

Range:
7.8 - 500 pmol/L

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 100% 82%-103%

Product overview:
KE00045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The INS ELISA kit is to be used to detect and quantify protein levels of endogenous INS. The assay recognizes human INS. A monoclonal antibody specific for INS has been pre-coated onto the microwells. The INS protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for INS is added to detect the captured INS protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
ILPR, INS, insulin, IRDN
Background

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.


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