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Human JNK ELISA Kit0 Publications

Catalog number: KE00072

规格

单价

96 T

￥2600

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Overview

Product name:
Human JNK ELISA Kit
Tests:
1 X 96 well plate
Sample type:
Cell lysate
Assay type:
Sandwich
Sensitivity:
0.1 ng/mL
Range:
1.56-100 ng/mL
Reacted Species:
Human
Tested applications:
Sandwich ELISA
Recovery:
Sample TypeAverageRange
Cell lysate 100% 85%-110%
IntraAssay:
SamplenmeanSDCV%
1 20 82 5.5 6.7
2 20 28.5 0.8 2.8
3 20 6.3 0.3 5.3
InterAssay:
SamplenmeanSDCV%
1 24 86 6.3 7.3
2 24 28 2 7.2
3 24 5.5 0.5 8.9
Product overview:
KE00072 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The JNK ELISA kit is designed for the quantitative determination of JNK in samples of human and mouse origin. A polyclonal antibody specific for JNK has been pre-coated onto the microwells. The JNK protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for JNK is added to detect the captured JNK protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties

Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:
c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, JNK-phospho-Thr183/Tyr185, JNK-phospho-Tyr185, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1
Background

JNK is also named as MAPK8 (Mitogen-activated protein kinase 8), PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. JNK is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. JNK functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. JNK activity is abnormally elevated in obesity. Furthermore, an absence of JNK results in decreased adiposity, significantly improved insulin sensitivity, and enhanced insulin receptor signaling capacity in 2 different models of mouse obesity, including ob/ob. JNK is a crucial mediator of obesity and insulin resistance and a potential target for therapeutics. The ELISA kit is suitable for testing cell lysates.


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