Human PD1 ELISA Kit

Catalog number: KE00075

规格

单价

96 T

¥2600

Images See all

Overview


Product name:
Human PD1 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant,Cell lysate

Assay type:
Sandwich

Sensitivity:
43 pg/mL

Range:
125-8000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 96% 80%-116%
Cell culture supernatant 95% 79%-120%
Cell lysate 101% 83%-125%

Product overview:
KE00075 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PD1 ELISA kit is to be used to detect and quantify protein levels of endogenous PD1. The assay recognizes human PD1. A polyclonal antibody specific for PD1 has been pre-coated onto the microwells. The PD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for PD1 is added to detect the captured PD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
CD279, hPD 1, hPD l, PD1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Background

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 can be expressed on T cells, B cells, natural killer T cells, activated monocytes, and dendritic cells (DCs). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity.


Back
to top