Human SERPINA3 ELISA Kit

Catalog number: KE00037

规格

单价

96 T

¥2600

Images See all

Overview


Product name:
Human SERPINA3 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
serum, plasma

Assay type:
Sandwich

Sensitivity:
0.8 ng/mL

Range:
1-64 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 99% 82%-113%

Product overview:
KE00037 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA3 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA3. The assay recognizes human SERPINA3. A polyclonal antibody specific for SERPINA3 has been pre-coated onto the microwells. The SERPINA3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for SERPINA3 is added to detect the captured SERPINA3 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
A1 Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3, SERPINA3,AACT
Background

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson’s disease (PD), Alzheimer’s disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.


Back
to top