Human TNFR1 ELISA Kit

Catalog number: KE00051

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Overview


Product name:
Human TNFR1 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Urine

Assay type:
Sandwich

Sensitivity:
2.9 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 89% 76%-98%
Cell culture supernatant 101% 90%-116%
Urine 102% 85%-111%

Product overview:
KE00051 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFR1 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFR1. The assay recognizes human TNFR1. A polyclonal antibody specific for TNFR1 has been pre-coated onto the microwells. The TNFR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TNFR1 is added to detect the captured TNFR1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A
Background

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55-kDa TNFR1. Soluble TNFR1 could function as TNF-binding protein, inhibiting TNF bioactivity.


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