Human PGⅡ ELISA Kit

Catalog number: KE00090

规格

单价

96 T

¥2600

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Overview


Product name:
Human PGⅡ ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.03 ng/mL

Range:
1.56 - 100 ng/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 99% 75%-128%
Cell culture supernatant 92% 86%-101%

Product overview:
KE00090 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGⅡ ELISA kit is to be used to detect and quantify protein levels of endogenous PGⅡ. The assay recognizes human PGⅡ. A polyclonal antibody specific for PGⅡ has been pre-coated onto the microwells. The PGⅡ protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for PGⅡ is added to detect the captured PGⅡprotein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
Store standard and Plate at -20° the rests store at 4°.Refer to the protocol for further storage instructions

Synonyms:
Gastricsin, Pepsinogen C, Pepsinogen II, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C)
Background

PepsinogenⅠand Ⅱ, as precursors of pepsin, are produced by gastric mucosa and released into the gastric lumen and peripheral circulation. The advanced inflammation of gastric mucosa and its progression toward atrophic gastritis (AG) in the corpus is associated with a change in serum biomarkers pepsinogenⅠand Ⅱ.(PMID:23496362). PepsinogenⅡ also known as progastricsin or PGC (pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis.


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