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  • KD/KO Validated

IFITM1-Specific Monoclonal antibody

IFITM1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5B5E2

Cat No : 60074-1-Ig

Print datasheet

Synonyms

9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13



经过测试的应用

Positive WB detected inTF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inK-562 cells
Positive FC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60074-1-Ig targets IFITM1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications FC, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen IFITM1-Specific fusion protein Ag2320 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interferon induced transmembrane protein 1 (9-27)
Synonyms 9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC000897
Gene Symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2233405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13164
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

实验方案

Product Specific Protocols
WB protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IHC protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IF protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IP protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
FC protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,FC

Science

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Authors - Julian Buchrieser
humanWB

Cell

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Authors - Abraham L Brass
humanWB

Cell Stem Cell

PMID: 30416070

Authors - Carolina Petrillo
humanWB

Sci Transl Med

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Authors - Mohammed Selman
humanWB

Cell Host Microbe

Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.

Authors - Toshana L Foster
  • KD Validated
humanIF

Gastroenterology

PMID: 23123437

Authors - Daryl T-Y Lau