IL-1 Beta Monoclonal antibody
IL-1 Beta Monoclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (2)
Applications
IHC, IP, WB,ELISA
Conjugate
Unconjugated
CloneNo.
1C6F12
验证数据展示
经过测试的应用
Positive WB detected in | Recombinant protein |
Positive IP detected in | RAW 264.7 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 12 publications below |
ELISA | See 1 publications below |
产品信息
60136-1-Ig targets IL-1 Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IP, WB,ELISA |
Cited Applications | ELISA, WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | IL-1 Beta fusion protein Ag10295 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | interleukin 1, beta |
Synonyms | |
Calculated Molecular Weight | 269 aa, 31 kDa |
GenBank Accession Number | BC008678 |
Gene Symbol | IL1B |
Gene ID (NCBI) | 3553 |
ENSEMBL Gene ID | ENSG00000125538 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P01584 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IL-1 Beta antibody 60136-1-Ig | Download protocol |
IHC protocol for IL-1 Beta antibody 60136-1-Ig | Download protocol |
IP protocol for IL-1 Beta antibody 60136-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway. | ||
Nat Cell Biol Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity. | ||
Nat Commun CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. | ||
Oncotarget Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway. | ||
J Ethnopharmacol Yi-shen-hua-shi granules modulate immune and inflammatory damage via the ALG3/PPARγ/NF-κB pathway in the treatment of immunoglobulin a nephropathy |