CD11c/Integrin Alpha X Polyclonal antibody

CD11c/Integrin Alpha X Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17342-1-AP

Print datasheet

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6



经过测试的应用

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17342-1-AP targets CD11c/Integrin Alpha X in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, pig
Immunogen CD11c/Integrin Alpha X fusion protein Ag11380 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Synonyms CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6
Calculated Molecular Weight 1169 aa, 129 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC038237
Gene Symbol ITGAX
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
RRIDAB_2129787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

实验方案

Product Specific Protocols
WB protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
IHC protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
FC protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
IHC

Acta Pharm Sin B

Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice.

Authors - Yuyu Zhu
FC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang
humanIF

J Immunother Cancer

TSH-TSHR axis promotes tumor immune evasion.

Authors - Zhenghao Wu
FC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
IF

Theranostics

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Authors - Ke Feng