JUNB Polyclonal antibody

JUNB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10486-1-AP

Print datasheet

Synonyms

AP 1, jun B proto oncogene, JUNB, Transcription factor jun B



经过测试的应用

Positive WB detected inMCF7 cells, C6 cells, HeLa cells, HT-1080 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10486-1-AP targets JUNB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen JUNB fusion protein Ag0752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name jun B proto-oncogene
Synonyms AP 1, jun B proto oncogene, JUNB, Transcription factor jun B
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 42 kDa, 43 kDa
GenBank Accession NumberBC004250
Gene Symbol JUNB
Gene ID (NCBI) 3726
RRIDAB_2129996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17275
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

实验方案

Product Specific Protocols
WB protocol for JUNB antibody 10486-1-APDownload protocol
IHC protocol for JUNB antibody 10486-1-APDownload protocol
IF protocol for JUNB antibody 10486-1-APDownload protocol
IP protocol for JUNB antibody 10486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
mouseWB

J Exp Med

USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.

Authors - Siyuan Chen
mouse,ratWB

Pharmacol Res

Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.

Authors - Jiale Cai
humanWB

Am J Cancer Res

PMID: 31285951

Authors - Wen-Si Zhao
humanWB

J Proteome Res

Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.

Authors - Bing Wang
humanWB

Exp Cell Res

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1

Authors - Xuanxuan Hu