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  • KD/KO Validated

TIP60/KAT5 Polyclonal antibody

TIP60/KAT5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 10827-1-AP

Print datasheet

Synonyms

60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60



经过测试的应用

Positive WB detected inPC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KAT5 Ready-To-Use IHC Kit. KAT5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10827-1-AP targets TIP60/KAT5 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsChIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TIP60/KAT5 fusion protein Ag1261 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name K(lysine) acetyltransferase 5
Synonyms 60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60
Calculated Molecular Weight 60 kDa
Observed Molecular Weight58 kDa, 70 kDa
GenBank Accession NumberBC000166
Gene Symbol KAT5
Gene ID (NCBI) 10524
RRIDAB_2128431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92993
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa

实验方案

Product Specific Protocols
WB protocol for TIP60/KAT5 antibody 10827-1-APDownload protocol
IHC protocol for TIP60/KAT5 antibody 10827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

Authors - Arnaud Obri
mouseWB

Nat Immunol

The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells.

Authors - Jing Geng

Mol Cell

PMID: 28366643

Authors - Sophie Barral
humanWB

Nat Commun

Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.

Authors - Zhiyuan You
  • KD Validated
humanIF,IP

J Mol Cell Biol

Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis

Authors - Hameed Akbar
humanWB

Mol Cell Proteomics

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Authors - Heguo Yu