KIF5C Polyclonal antibody
KIF5C Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 25897-1-AP is KIF5C Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | CoIP, IHC, WB |
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse |
Immunogen | KIF5C fusion protein Ag23163 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | kinesin family member 5C |
Synonyms | KIAA0531, KIF5C, kinesin family member 5C, Kinesin heavy chain isoform 5C, KINN, NKHC, NKHC 2, NKHC2 |
Calculated Molecular Weight | 725 aa, 83 kDa |
Observed Molecular Weight | 70 kDa, 110 kDa |
GenBank Accession Number | BC110287 |
Gene Symbol | KIF5C |
Gene ID (NCBI) | 3800 |
RRID | AB_2880288 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60282 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for KIF5C antibody 25897-1-AP | Download protocol |
IHC protocol for KIF5C antibody 25897-1-AP | Download protocol |
IF protocol for KIF5C antibody 25897-1-AP | Download protocol |
IP protocol for KIF5C antibody 25897-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Exp Neurol Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI | ||
Hum Mol Genet Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients. | ||
Alzheimers Res Ther Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport. | ||
bioRxiv MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A | ||
Int Endod J Overexpression of MicroRNA-155 Aggravates Pulpitis by Targeting Kinesin Superfamily Proteins-5C Based on Illumina High-Throughput Sequencing
| ||
Exp Mol Med Activation of TLR7-mediated autophagy increases epileptic susceptibility via reduced KIF5A-dependent GABAA receptor transport in a murine model |