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Cytokeratin 15 Monoclonal antibody

Cytokeratin 15 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

6B4F8

Cat No : 60247-1-Ig

Print datasheet

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

验证数据展示

  • WB analysis of A431 using 60247-1-Ig

    WB analysis of A431 using 60247-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 60247-1-Ig

    IHC staining of human oesophagus cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens).

    Ready-to-use IHC kits available with KRT15 antibody included
  • IHC staining of human oesophagus cancer using 60247-1-Ig

    IHC staining of human oesophagus cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens).

    Ready-to-use IHC kits available with KRT15 antibody included
  • IF Staining of HeLa using 60247-1-Ig

    IF Staining of HeLa using 60247-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of A431 using 60247-1-Ig

    FC experiment of A431 using 60247-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (60247-1-Ig, Clone:6B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 60247-1-Ig is Cytokeratin 15 Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Immunogen Cytokeratin 15 fusion protein Ag0185 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 15
Synonyms CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002641
Gene Symbol KRT15
Gene ID (NCBI) 3866
RRIDAB_2881369
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19012
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IHC protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IF protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Oxid Med Cell Longev

Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway

Authors - Jiali Li
View Article