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- KD/KO Validated
LACTB Polyclonal antibody
LACTB Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human heart tissue, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18195-1-AP targets LACTB in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | LACTB fusion protein Ag12233 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lactamase, beta |
| Synonyms | G24, lactamase, beta, LACTB, MRPL56 |
| Calculated Molecular Weight | 547 aa, 61 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC067288 |
| Gene Symbol | LACTB |
| Gene ID (NCBI) | 114294 |
| RRID | AB_2280998 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P83111 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LACTB is also named as MRPL56,which is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form. This antibody is specific to LACTB.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LACTB antibody 18195-1-AP | Download protocol |
| IHC protocol for LACTB antibody 18195-1-AP | Download protocol |
| IF protocol for LACTB antibody 18195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene LACTB, a novel epigenetic silenced tumor suppressor, inhibits colorectal cancer progression by attenuating MDM2-mediated p53 ubiquitination and degradation.
| ||
PLoS One LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis. | ||
Cancer Biother Radiopharm Clinical Significance of β-Lactamase Expression in Colorectal Cancer. | ||
Exp Ther Med LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway.
| ||
Apoptosis LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer |
